4MDK
Cdc34-ubiquitin-CC0651 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 1 |
Unit cell lengths | 55.403, 72.220, 75.912 |
Unit cell angles | 71.89, 79.63, 81.61 |
Refinement procedure
Resolution | 44.627 - 2.610 |
R-factor | 0.2069 |
Rwork | 0.204 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2OB4 AND 3A33 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.264 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.079 | 0.044 | 0.457 |
Number of reflections | 31394 | ||
<I/σ(I)> | 13.7 | 34.3 | 1.2 |
Completeness [%] | 95.8 | 97.2 | 85.5 |
Redundancy | 2.2 | 2.2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 28% PEG3350, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |