4MDE
Structure of bacterial polynucleotide kinase product complex bound to GDP and DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 130 |
Detector technology | PIXEL |
Collection date | 2013-08-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.440, 73.120, 119.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.995 - 1.800 |
R-factor | 0.1675 |
Rwork | 0.164 |
R-free | 0.20560 |
Structure solution method | molecular refinement |
RMSD bond length | 0.006 |
RMSD bond angle | 1.084 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.200 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 43723 | |
Completeness [%] | 97.8 | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 100 mM sodium citrate, pH 5.0, 2 mM guanosine diphosphate, 100mM magnesium chloride,18-24% PEG 6000,1.1 mM short DNA strand , VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |