4MDB
Structure of Mos1 transposase catalytic domain and Raltegravir with Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 44.240, 44.240, 206.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.290 - 1.700 |
| R-factor | 0.19928 |
| Rwork | 0.197 |
| R-free | 0.24076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f7t |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.302 |
| Number of reflections | 23539 | |
| <I/σ(I)> | 14.5 | 4.7 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 290 | 22% w/v PEG4000, 100 mM Tris, pH 6.8, 5 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
