4MAO
RSK2 T493M C-Terminal Kinase Domain in Complex with RMM58
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999974 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 47.500, 47.500, 288.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.117 - 2.600 |
R-factor | 0.2378 |
Rwork | 0.235 |
R-free | 0.29190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.992 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 10976 | |
Completeness [%] | 97.5 | 92.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM HEPES, 50 mM Ammonium Sulfate, 7.5% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |