4M9Z
Crystal structure of CED-4 bound CED-3 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97894 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 178.167, 178.167, 200.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.212 - 3.405 |
| R-factor | 0.2724 |
| Rwork | 0.270 |
| R-free | 0.32710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lqq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.533 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 |
| High resolution limit [Å] | 3.405 | 3.405 |
| Number of reflections | 48180 | |
| Completeness [%] | 99.9 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.6-0.8M sodium acetate, 0.1M HEPES (pH 7.5), 0.1M sodium fluoride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
