4M91

crystal structure of hN33/Tusc3-peptide 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	39.769, 62.235, 64.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.880 - 1.100
R-factor	0.1378
Rwork	0.137
R-free	0.16023
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m8g
RMSD bond length	0.015
RMSD bond angle	1.594
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.770
High resolution limit [Å]	1.100
Number of reflections	65763
<I/σ(I)>	18.2
Completeness [%]	96.2
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5		0.2 M KSCN, 10.5% PEG 8K, 10% PEG 1K in 100 mM cacodylic acid-NaOH, pH 6.5, VAPOR DIFFUSION, SITTING DROP