4M8Q
Ontogeny of recognition specificity and functionality for the anti-HIV neutralizing antibody 4E10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-17 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.561, 77.674, 77.823 |
| Unit cell angles | 90.00, 112.03, 90.00 |
Refinement procedure
| Resolution | 24.610 - 2.890 |
| R-factor | 0.26294 |
| Rwork | 0.261 |
| R-free | 0.30168 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.610 | 2.950 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Number of reflections | 17941 | |
| Completeness [%] | 99.6 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M KCl, 12% w/v PEG 8000, 5% v/v glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
