4M78
Crystal structure of Lsm2-8 complex, space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-08 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.721, 76.638, 113.554 |
Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
Resolution | 29.068 - 2.794 |
R-factor | 0.2555 |
Rwork | 0.254 |
R-free | 0.29360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m75 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.376 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.794 | 2.794 |
Number of reflections | 33539 | |
Completeness [%] | 98.2 | 83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |