4M76
Integrin I domain of complement receptor 3 in complex with C3d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.460, 65.120, 62.540 |
| Unit cell angles | 90.00, 115.69, 90.00 |
Refinement procedure
| Resolution | 45.107 - 2.804 |
| R-factor | 0.1979 |
| Rwork | 0.194 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1IDO 1c3d |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.657 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.895 | 3.000 | |
| High resolution limit [Å] | 2.800 | 50.000 | 2.800 |
| Rmerge | 0.049 | 0.291 | |
| Number of reflections | 12888 | ||
| <I/σ(I)> | 16.77 | 4.47 | |
| Completeness [%] | 98.1 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 3350, pH 7, vapor diffusion, hanging drop, temperature 298K |
