4M6R
Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000, 0.91977, 0.91957, 0.90633 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.035, 107.735, 192.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18294 |
| Rwork | 0.181 |
| R-free | 0.22027 |
| Structure solution method | MAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.050 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 72069 | |
| Completeness [%] | 96.0 | 81.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 285 | 0.04M citric acid, 0.06M bis-tris propane, 5%(v/v) glycerol, 21%(w/v) PEG 3350, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
