4M6O
Crystal structure of anti-NGF antibody CNTO7309
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-08-10 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 3 |
Unit cell lengths | 152.600, 152.600, 152.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.18877 |
Rwork | 0.186 |
R-free | 0.25047 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1VGE AND 2AAB |
RMSD bond length | 0.007 |
RMSD bond angle | 1.342 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.086 | 0.405 |
Number of reflections | 13878 | |
<I/σ(I)> | 14.5 | 1.8 |
Completeness [%] | 95.7 | 61.7 |
Redundancy | 3.4 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES, pH 6.5, 12% PEG20000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |