4M69
Crystal structure of the mouse RIP3-MLKL complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 106.443, 141.951, 107.129 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.569 - 2.497 |
R-factor | 0.2215 |
Rwork | 0.220 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4M66 and 4M68 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.416 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.590 |
High resolution limit [Å] | 2.497 | 2.500 |
Number of reflections | 28489 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1M HEPES, 0.35M (NH4)2S04, 17% PEG4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |