4M64
3D crystal structure of Na+/melibiose symporter of Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 32 |
| Unit cell lengths | 127.206, 127.206, 206.303 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.640 - 3.350 |
| R-factor | 0.31422 |
| Rwork | 0.312 |
| R-free | 0.35945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Threading Model of MelBst on FucP |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.771 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.092 | 0.770 |
| Number of reflections | 52990 | |
| <I/σ(I)> | 6 | 1.52 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES, 0.1M NaCl, 0.05M CaCl2, 32% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
