4M61

Crystal structure of unliganded anti-DNA Fab A52

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-03-28
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	52.784, 61.380, 121.223
Unit cell angles	90.00, 96.51, 90.00

Refinement procedure

Resolution	21.694 - 1.620
R-factor	0.1959
Rwork	0.192
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mrf
RMSD bond length	0.008
RMSD bond angle	1.182
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.046	0.347
Number of reflections	94152
<I/σ(I)>	28.7	1.9
Completeness [%]	100.0	95.4
Redundancy	3.4	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	1.4 M ammonium sulfate, 0.07 M sodium citrate, 2% PEG400, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K