4M55
Crystal structure of Human UDP-xylose synthase R236H substitution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.171, 85.145, 292.549 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.312 - 2.860 |
R-factor | 0.2311 |
Rwork | 0.228 |
R-free | 0.28480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.123 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 42.300 | 2.960 |
High resolution limit [Å] | 2.860 | 8.190 | 2.860 |
Rmerge | 0.124 | 0.044 | 1.830 |
Number of reflections | 52758 | ||
<I/σ(I)> | 11.6 | 34.88 | 1.2 |
Completeness [%] | 99.1 | 99.11 | |
Redundancy | 6.15 | 5.5 | 5.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.95 | 298 | 0.5 M ammonium sulfate, 0.5 M sodium acetate, 0.1 M imidazole, 0.1 M HCl, pH 5.95, VAPOR DIFFUSION, HANGING DROP, temperature 298K |