4M4R
Epha4 ectodomain complex with ephrin a5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 207.541, 46.694, 262.305 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.130 |
| R-factor | 0.24661 |
| Rwork | 0.244 |
| R-free | 0.29044 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wo3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.240 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.669 | |
| Number of reflections | 84233 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.2 | 96.4 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 2.0 M Sodium Acetate, 0.1 M Sodium Acetate buffer pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
