4M2B

Crystal structure of L281D mutant of udp-glucose pyrophosphorylase from leishmania major in complex with udp-glc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-25
Detector	MAR CCD 165 mm
Spacegroup name	C 2 2 21
Unit cell lengths	78.790, 86.900, 138.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.400 - 2.200
R-factor	0.178
Rwork	0.175
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oeg
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	23.400
High resolution limit [Å]	2.200
Rmerge	0.158
Number of reflections	23985
<I/σ(I)>	14.4
Completeness [%]	98.0
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.6	277	0.1M BIS-TRIS, 28% W/V PEG-MME -2000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K, pH 6.6