4M0Q

Ebola virus VP24 structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790128
Spacegroup name	P 43 21 2
Unit cell lengths	71.775, 71.775, 191.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.597 - 1.921
R-factor	0.2107
Rwork	0.209
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d9o
RMSD bond length	0.010
RMSD bond angle	1.534
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.920	5.210	1.920
Rmerge	0.089	0.051	0.963
Number of reflections	39161
<I/σ(I)>	7.6
Completeness [%]	100.0	99.5	100
Redundancy	9.6	8.6	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	150 mM MES, pH 6.4, 20% Jeffamine M-600, pH 7.0, 50 mM cesium chloride, 6 mM barium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K