4M0L

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus complexed with GDP

Replaces:  3P3M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	CCD
Collection date	2012-01-18
Detector	Bruker Platinum 135
Wavelength(s)	1.54179
Spacegroup name	P 1 21 1
Unit cell lengths	86.050, 106.550, 156.300
Unit cell angles	90.00, 90.63, 90.00

Refinement procedure

Resolution	19.811 - 2.600
R-factor	0.2364
Rwork	0.234
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qn6
RMSD bond length	0.005
RMSD bond angle	0.965
Data reduction software	PROTEUM PLUS (PLUS)
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.811	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.056	0.444
Number of reflections	84101
<I/σ(I)>		1.93
Completeness [%]	96.7	90.5
Redundancy	3.27	2.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	100mM Tris-HCl, 50mM NaCl, 50mM KCl, 5mM MgCl2, 5mM 2-mercaptoethanol, 90mM KBr, 7.5% PEG 4000, 0.5mM GTP, VAPOR DIFFUSION, HANGING DROP, temperature 295K