4LZZ
Nucleotide-induced asymmetry within atpase activator ring drives s54-RNAP interaction and ATP hydrolysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 119.970, 130.830, 208.230 |
Unit cell angles | 90.01, 90.01, 89.85 |
Refinement procedure
Resolution | 44.269 - 3.210 |
R-factor | 0.2676 |
Rwork | 0.267 |
R-free | 0.32230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.127 |
Data reduction software | d*TREK (9.7 W8RSSI) |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.270 | 44.270 | 2.970 |
High resolution limit [Å] | 2.870 | 6.180 | 2.870 |
Rmerge | 0.118 | 0.032 | 0.511 |
Number of reflections | 256925 | ||
<I/σ(I)> | 3 | 12.1 | 0.8 |
Completeness [%] | 88.7 | 90.3 | 76.1 |
Redundancy | 1.76 | 1.81 | 1.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 277 | 20% ethylene glycol, crystal soaked in fresh mother liquor prior to flash cooling, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |