4LZJ
Crystal Structure of MurQ from H.influenzae with bound inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 76.492, 113.335, 143.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.520 - 2.405 |
R-factor | 0.1687 |
Rwork | 0.166 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nri |
RMSD bond length | 0.008 |
RMSD bond angle | 1.197 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.520 |
High resolution limit [Å] | 2.400 |
Number of reflections | 48045 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M NaCl, 0.1 M Bis-Tris:HCl, pH 5.5, 25% (w/v) PEG-3350, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |