4LWZ

Crystal structure of Myo5b globular tail domain in complex with inactive Rab11a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.980110
Spacegroup name	P 21 21 21
Unit cell lengths	67.680, 143.140, 162.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.968 - 2.550
R-factor	0.2342
Rwork	0.231
R-free	0.29530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.003
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.620
High resolution limit [Å]	2.550	2.550
Number of reflections	52120
Completeness [%]	99.5	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	17% peg, 100 mM NaCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K