4LW2

Structural changes during cysteine desulfurase CsdA and sulfur-acceptor CsdE interactions provide insight into the trans-persulfuration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-22
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	168.298, 91.441, 83.894
Unit cell angles	90.00, 97.26, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.16713
Rwork	0.165
R-free	0.21235
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c0n
RMSD bond length	0.028
RMSD bond angle	2.108
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.470	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	116275
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	20-30% (w/v) PEG 8000, 0.05M potassium di-hydrogen phosphate, 0.1M MES, 0.1M Tris, pH 5.5-6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K