4LT6
Crystal Structure of human poly(A) polymerase gamma
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-19 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.870, 89.920, 201.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.884 - 2.790 |
R-factor | 0.1962 |
Rwork | 0.193 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q78 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.250 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.970 |
High resolution limit [Å] | 2.790 | 5.060 | 2.790 |
Number of reflections | 31418 | ||
Completeness [%] | 98.1 | 99.7 | 89.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 20%(W/V) PEG 8000, 50mM Tris-HCl, 100mM (NH4)2SO4, 5mM CaCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |