4LSU
Crystal structure of broadly and potently neutralizing antibody VRC-PG20 in complex with HIV-1 clade A/E 93TH057 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-16 |
| Detector | MAR CCD 300 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.283, 66.313, 95.108 |
| Unit cell angles | 90.00, 108.34, 90.00 |
Refinement procedure
| Resolution | 45.140 - 2.300 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.393 |
| Number of reflections | 39655 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 90.7 | 44.6 |
| Redundancy | 3.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.1 M HEPES, pH 7.0, 9% PEG 4000, 8% isopropanol, 0.01M CaCl2,, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
