4LRQ
Crystal structure of a Low Molecular Weight Phosphotyrosine phosphatase from Vibrio choleraeO395
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-12-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 87.470, 87.470, 73.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.410 - 1.450 |
| R-factor | 0.15204 |
| Rwork | 0.152 |
| R-free | 0.16063 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.959 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.890 | 1.488 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.057 | |
| Number of reflections | 110187 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 2.4M ammonium sulfate, 0.1M citric acid pH 5.0, 2% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
