4LQI
Yeast 20S Proteasome in complex with Vibralactone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.860, 299.400, 144.990 |
| Unit cell angles | 90.00, 113.07, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.700 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.113 | 0.523 |
| Number of reflections | 289175 | |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20 mM magnesium acetate, 100 mM MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
