4LOT
C1s CUB2-CCP1-CCP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.040, 58.590, 51.210 |
| Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
| Resolution | 54.217 - 2.920 |
| R-factor | 0.2658 |
| R-free | 0.29230 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.959 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.220 | 2.950 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 9420 | |
| Completeness [%] | 94.8 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 24% PEG 8K in Bis-tris propane-HCL, containing 200 mM potassium citrate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
