4LNP
The first SH3 domain from CAP/Ponsin in complex with proline rich peptide from Vinculin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 77 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.480, 49.850, 58.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.060 - 1.410 |
R-factor | 0.13954 |
Rwork | 0.137 |
R-free | 0.19361 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 2.394 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.060 |
High resolution limit [Å] | 1.410 |
Number of reflections | 13539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 293 | pH 8.0, EVAPORATION, temperature 293K |