4LMQ
Development and Preclinical Characterization of a Humanized Antibody Targeting CXCL12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2012-03-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.95369 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.949, 73.101, 106.626 |
Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
Resolution | 55.296 - 2.773 |
R-factor | 0.255 |
Rwork | 0.253 |
R-free | 0.30070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.717 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.000 | 2.780 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 25088 | |
Completeness [%] | 98.1 | 87.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.2M NaF, 0.1M Bis-tris, propane, 23%w/v PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |