4LMB
Crystal structure analysis of O-acetylserine sulfhydrylase CysK2 complexed with cystine from Microcystis aeruginosa 7806
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 99.846, 99.846, 164.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.060 - 1.910 |
| R-factor | 0.20308 |
| Rwork | 0.202 |
| R-free | 0.23028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.304 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 1.910 | 2.300 |
| Number of reflections | 36107 | |
| Completeness [%] | 99.1 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 1.5M (NH4)2SO4, 0.1M Bis-Tris-propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
