4LLY
Crystal structure of Pertuzumab Clambda Fab with variable and constant domain redesigns (VRD2 and CRD2) at 1.6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Collection date | 2013-02-11 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | .97931 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.772, 52.847, 89.483 |
| Unit cell angles | 76.88, 84.32, 85.53 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.19931 |
| Rwork | 0.198 |
| R-free | 0.22929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.384 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 118271 | |
| Completeness [%] | 95.9 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 100mM Na Citrate tribasic dihydrate pH 5 + 30% Jeffamine ED-2001 , VAPOR DIFFUSION, SITTING DROP, temperature 294K |
