4LLU
Structure of Pertuzumab Fab with light chain Clambda at 2.16A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-10-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | .97931 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 210.024, 69.739, 70.828 |
Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.160 |
R-factor | 0.22501 |
Rwork | 0.222 |
R-free | 0.27279 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s78 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.328 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.902 | 2.280 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.175 | 0.670 |
Number of reflections | 52049 | |
Completeness [%] | 97.4 | 94.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 100mM Sodium Acetate pH 4.5 + 10% MPD + 30% PEG 2000 MME + 200mM Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |