4LLD
Structure of wild-type IgG1 antibody heavy chain constant domain 1 and light chain lambda constant domain (IgG1 CH1:Clambda) at 1.19A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 77 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-23 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | .97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.080, 65.837, 66.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.934 - 1.190 |
R-factor | 0.14596 |
Rwork | 0.145 |
R-free | 0.16992 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3thm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.286 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.925 | 1.250 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmerge | 0.043 | 0.528 |
Number of reflections | 60110 | |
Completeness [%] | 97.6 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 35% PEG 4K, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |