4LJO
Structure of an active ligase (HOIP)/ubiquitin transfer complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.920 |
Spacegroup name | P 31 |
Unit cell lengths | 45.950, 45.950, 133.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.337 - 1.564 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ljq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.032 |
Data reduction software | CCP4 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.340 | 1.600 | |
High resolution limit [Å] | 1.560 | 6.980 | 1.560 |
Rmerge | 0.039 | 0.040 | 0.593 |
Number of reflections | 44055 | ||
<I/σ(I)> | 12.2 | 16.71 | 1.52 |
Completeness [%] | 99.2 | 88.5 | 94 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M carboxylic acids, 0.1 M imidazole, MES, 30 % P550 MME_P20K, pH 6.5, vapour diffusion, temperature 293K, VAPOR DIFFUSION |