4LIT

Structure of YcfD a Ribosomal oxygenase from Escherichia coli in complex with Cobalt and 2-oxoglutarate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-18
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	74.140, 74.140, 208.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.156 - 2.400
R-factor	0.1864
Rwork	0.184
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4liu
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.869	33.156	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.057	0.730
Total number of observations		9782	42271
Number of reflections	23748
<I/σ(I)>	11.8	8.2	1.1
Completeness [%]	100.0	99	100
Redundancy	12.9	11.1	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	285	10mM Cobalt chloride, 50mM 2-oxoglutarate pH 4.2, 1.5M 1,6 Hexanediol, VAPOR DIFFUSION, HANGING DROP, temperature 285K