4LIF
Crystal structure of the JCV large T-antigen origin binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | I 41 |
Unit cell lengths | 103.793, 103.793, 34.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.696 - 2.600 |
R-factor | 0.1782 |
Rwork | 0.176 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fuf |
RMSD bond length | 0.007 |
RMSD bond angle | 0.990 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.068 |
Number of reflections | 5906 |
<I/σ(I)> | 37.6 |
Completeness [%] | 99.8 |
Redundancy | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 273 | 0.1 M Tris, 0.2 M LiSO4, 30 % Peg 4000, VAPOR DIFFUSION, SITTING DROP, temperature 273K, pH 8.5 |