4LHW

Crystal structure of Rab8 in its active GppNHp-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-01
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	90.381, 96.676, 105.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 1.550
R-factor	0.146
Rwork	0.144
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yzq
RMSD bond length	0.013
RMSD bond angle	1.479
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.900
High resolution limit [Å]	1.550
Number of reflections	132605
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	Rab8a6-176:GppNHp (15 mg/ml; buffer: 25 mM HEPES, 40 mM NaCl, 1 mM MgCl2, 10 M GppNHp and 5 mM -mercaptoethanol) crystals were produced in 15% (w/v) PEG8000, 7.5 %(v/v) MPD, 0.1 M HEPES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K