4LHM

Thymidine phosphorylase from E.coli with 3'-azido-3'-deoxythymidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-23
Wavelength(s)	0.8
Spacegroup name	P 43 21 2
Unit cell lengths	130.762, 130.762, 67.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.000 - 1.520
R-factor	0.15537
Rwork	0.153
R-free	0.19578
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.250
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.520
Number of reflections	84887
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		298	COUNTER DIFFUSION, temperature 298.0K