4LH0

Structure of mouse 1-Pyrroline-5-Carboxylate Dehydrogenase (ALDH4A1) complexed with glyoxylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	148
Detector technology	IMAGE PLATE
Collection date	2012-09-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	84.870, 94.242, 132.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.664 - 1.674
R-factor	0.16
Rwork	0.159
R-free	0.18350
RMSD bond length	0.006
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHENIX
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.668	19.668	1.760
High resolution limit [Å]	1.674	5.290	1.670
Rmerge		0.043	0.234
Total number of observations		19061	57767
Number of reflections	120763
<I/σ(I)>	14.9	11.8	3.2
Completeness [%]	98.7	97.7	91.9
Redundancy	4.5	4.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	15-25% PEG 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K