4LG9

Crystal structure of TBL1XR1 WD40 repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-20
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 31 2 1
Unit cell lengths	90.776, 90.776, 104.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.388 - 2.280
R-factor	0.1656
Rwork	0.163
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ymu ensemble of triple repeats (345-467 102-225)
RMSD bond length	0.007
RMSD bond angle	1.149
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (dev_1439)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.614	45.388	2.030
High resolution limit [Å]	1.930	6.100	1.930
Rmerge		0.051	0.979
Number of reflections	23135
<I/σ(I)>	12.7	13.3	0.8
Completeness [%]	100.0	99.8	100
Redundancy	9.3	10.5	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			vapor diffusion