4LFI
Crystal structure of scCK2 alpha in complex with GMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.97906 |
Spacegroup name | P 32 |
Unit cell lengths | 69.664, 69.664, 166.251 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.860 - 1.850 |
R-factor | 0.17202 |
Rwork | 0.171 |
R-free | 0.20046 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.266 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.850 |
Number of reflections | 77066 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 0.2M Lithium sulfate monohydrate, 0.1M BIS-TRIS pH 6.5, 25% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 287K |