4LF4

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	400.609, 400.609, 175.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.352 - 3.342
R-factor	0.1737
Rwork	0.172
R-free	0.20770
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	0.816
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.470
High resolution limit [Å]	3.342	7.200	3.342
Rmerge	0.096	0.038	0.802
Number of reflections	202709
<I/σ(I)>	12.3
Completeness [%]	99.1	94.6	99.9
Redundancy	3.2	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	277	MPD, pH 6.5, hanging drop, temperature 277K