4LEX
Unliganded crystal structure of mAb7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 59.069, 84.184, 88.959 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.209 - 2.020 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fvd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.110 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
High resolution limit [Å] | 2.020 | 4.350 | 2.020 |
Rmerge | 0.090 | 0.069 | 0.219 |
Number of reflections | 27181 | ||
<I/σ(I)> | 11.3 | ||
Completeness [%] | 90.9 | 85.4 | 92.8 |
Redundancy | 5.3 | 5.3 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES, 30.0% Jeffamine ED-2001 (v/v) at 15 mg/mL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |