4LDZ
Crystal structure of the full-length response regulator DesR in the active state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-03-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.612, 77.646, 139.751 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.920 - 2.310 |
R-factor | 0.1907 |
Rwork | 0.189 |
R-free | 0.22140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 69.876 | 28.924 | 2.430 |
High resolution limit [Å] | 2.307 | 7.300 | 2.310 |
Rmerge | 0.018 | 0.506 | |
Total number of observations | 3014 | 12994 | |
Number of reflections | 22585 | ||
<I/σ(I)> | 15 | 32.1 | 1.5 |
Completeness [%] | 99.8 | 98.4 | 98.7 |
Redundancy | 4.1 | 3.7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 12% PEG3350, 0.2 M Sodium tartrate, 0.2 M MgCl2, 5 mM BeF3 and 5% (w/v) Methanol, vapor diffusion , pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |