4LD5
Crystal structure of MepR Q18P mutant from multidrug resistant S. aureus clinical isolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-20 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.842, 45.841, 210.060 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 34.929 - 2.400 |
| R-factor | 0.2132 |
| Rwork | 0.211 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.595 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHENIX ((Phaser)) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 51325 |
| Completeness [%] | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 0.2 M Na2SO4, 20% PEG 3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
