4LBQ
Crystal structure of mouse galectin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.459 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.040, 38.200, 130.180 |
| Unit cell angles | 90.00, 112.71, 90.00 |
Refinement procedure
| Resolution | 35.750 - 2.400 |
| R-factor | 0.1884 |
| Rwork | 0.186 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m2m |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.862 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.752 | 2.527 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.444 | 0.444 |
| Number of reflections | 19975 | |
| <I/σ(I)> | 4.5 | 2.5 |
| Completeness [%] | 99.9 | 99.88 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294 | 20% PEG 3350, 0.2 M ammonium fluoride, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
