4L9N

Crystal structure of MepR A103V mutant from multidrug resistant S. aureus clinical isolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	31.793, 95.125, 105.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.641 - 1.600
R-factor	0.1909
Rwork	0.190
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.908
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((Phaser))
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	41385
Completeness [%]	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.2 M (NH4)2SO4, 0.1 M Na acetate pH 4.6 and 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K