4L8I
Crystal structure of RSV epitope scaffold FFL_005
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.406, 53.406, 178.508 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.250 - 2.000 |
| R-factor | 0.2089 |
| Rwork | 0.207 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.555 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.067 | 0.038 | 0.392 |
| Number of reflections | 20776 | ||
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 99.3 | 99.3 | 94 |
| Redundancy | 7 | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 22% PEG 3350, 0.2M ammonium citrate tribasic, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
