4L71
Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.241, 446.100, 623.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.675 - 3.900 |
| R-factor | 0.237079404994 |
| Rwork | 0.235 |
| R-free | 0.27829 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3I9D AND 3I9E |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.201 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.676 | 3.800 | |
| High resolution limit [Å] | 3.700 | 16.550 | 3.700 |
| Rmerge | 0.313 | 0.014 | 0.014 |
| Number of reflections | 612965 | 6754 | 45309 |
| <I/σ(I)> | 4.51 | 27.79 | 1.08 |
| Completeness [%] | 99.1 | 90.6 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K |
